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recombinant human pad2 (rhpad2)  (Cayman Chemical)


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    Structured Review

    Cayman Chemical recombinant human pad2 (rhpad2)
    <t>PAD2</t> ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .
    Recombinant Human Pad2 (Rhpad2), supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+pad2+%28rhpad2%29/pmc08055403-53-0-7?v=Cayman+Chemical
    Average 90 stars, based on 1 article reviews
    recombinant human pad2 (rhpad2) - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination"

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    Journal: Journal of Immunology Research

    doi: 10.1155/2021/6659960

    PAD2 ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .
    Figure Legend Snippet: PAD2 ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .

    Techniques Used:

    Crossreactivity of chimeric anti-PAD2 antibodies with recombinant mouse PAD2. The values in the bracket indicates EC 50 values of antibodies against rhPAD2 and rmPAD2 enzymes. The enzyme-linked immunosorbent assay protocol is described .
    Figure Legend Snippet: Crossreactivity of chimeric anti-PAD2 antibodies with recombinant mouse PAD2. The values in the bracket indicates EC 50 values of antibodies against rhPAD2 and rmPAD2 enzymes. The enzyme-linked immunosorbent assay protocol is described .

    Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay

    Kinetics of binding of chimeric anti-PAD2 monoclonal antibodies (mAbs) with recombinant human PAD2.
    Figure Legend Snippet: Kinetics of binding of chimeric anti-PAD2 monoclonal antibodies (mAbs) with recombinant human PAD2.

    Techniques Used: Binding Assay, Bioprocessing, Recombinant

    Ability of anti-PAD2 antibodies to inhibit PAD2-mediated citrullination of N α -benzoyl-L-arginine ethyl ester (BAEE). S4, S10, S24, S108, S170, and S309 antibodies were incubated with recombinant human PAD2 (rhPAD2) and buffer (20 mM Tris-HCl (pH 7.6), 150 mM NaCl, and 1 mM dithiothreitol) in a total volume of 44 μ L for 30 min at 25°C. BAEE and CaCl 2 (volume: 6 μ L) were added simultaneously and the mixture was stirred well. The mixture comprised 5 nM rhPAD2, 10 mM BAEE, 1 mM CaCl 2 , and various concentrations of antibodies (0.1–600 nM). The mixture was incubated at 37°C for 4 h, and 12.5 μ L of 5 M perchloric acid was added to stop the reaction. The level of citrullinated BAEE was determined using a colorimetric assay system.
    Figure Legend Snippet: Ability of anti-PAD2 antibodies to inhibit PAD2-mediated citrullination of N α -benzoyl-L-arginine ethyl ester (BAEE). S4, S10, S24, S108, S170, and S309 antibodies were incubated with recombinant human PAD2 (rhPAD2) and buffer (20 mM Tris-HCl (pH 7.6), 150 mM NaCl, and 1 mM dithiothreitol) in a total volume of 44 μ L for 30 min at 25°C. BAEE and CaCl 2 (volume: 6 μ L) were added simultaneously and the mixture was stirred well. The mixture comprised 5 nM rhPAD2, 10 mM BAEE, 1 mM CaCl 2 , and various concentrations of antibodies (0.1–600 nM). The mixture was incubated at 37°C for 4 h, and 12.5 μ L of 5 M perchloric acid was added to stop the reaction. The level of citrullinated BAEE was determined using a colorimetric assay system.

    Techniques Used: Incubation, Recombinant, Colorimetric Assay

    Ability of anti-PAD2 antibodies to inhibit recombinant human PAD2- (rhPAD2-) mediated histone citrullination. The anti-PAD2 antibodies were mixed with rhPAD2 and buffer as described in the legend of at 25°C for 30 min. Histone (1.8 mg/mL) and CaCl 2 (1 mM) were added simultaneously and the mixture was incubated for 4 h at 37°C. Citrullinated histone was determined using a colorimetric assay.
    Figure Legend Snippet: Ability of anti-PAD2 antibodies to inhibit recombinant human PAD2- (rhPAD2-) mediated histone citrullination. The anti-PAD2 antibodies were mixed with rhPAD2 and buffer as described in the legend of at 25°C for 30 min. Histone (1.8 mg/mL) and CaCl 2 (1 mM) were added simultaneously and the mixture was incubated for 4 h at 37°C. Citrullinated histone was determined using a colorimetric assay.

    Techniques Used: Recombinant, Incubation, Colorimetric Assay

    Alanine scanning of epitopes along with their half-maximal effective concentration. EC 50 (ng/mL) values determined using enzyme-linked immunosorbent assay ∗ .
    Figure Legend Snippet: Alanine scanning of epitopes along with their half-maximal effective concentration. EC 50 (ng/mL) values determined using enzyme-linked immunosorbent assay ∗ .

    Techniques Used: Concentration Assay, Enzyme-linked Immunosorbent Assay, Residue



    Similar Products

    recombinant human pad2 (rhpad2)  (Cayman Chemical)
    90
    Cayman Chemical recombinant human pad2 (rhpad2)
    <t>PAD2</t> ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .
    Recombinant Human Pad2 (Rhpad2), supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+pad2+%28rhpad2%29/pmc08055403-53-0-7?v=Cayman+Chemical
    Average 90 stars, based on 1 article reviews
    recombinant human pad2 (rhpad2) - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    PAD2 ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: PAD2 ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques:

    Crossreactivity of chimeric anti-PAD2 antibodies with recombinant mouse PAD2. The values in the bracket indicates EC 50 values of antibodies against rhPAD2 and rmPAD2 enzymes. The enzyme-linked immunosorbent assay protocol is described .

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Crossreactivity of chimeric anti-PAD2 antibodies with recombinant mouse PAD2. The values in the bracket indicates EC 50 values of antibodies against rhPAD2 and rmPAD2 enzymes. The enzyme-linked immunosorbent assay protocol is described .

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

    Kinetics of binding of chimeric anti-PAD2 monoclonal antibodies (mAbs) with recombinant human PAD2.

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Kinetics of binding of chimeric anti-PAD2 monoclonal antibodies (mAbs) with recombinant human PAD2.

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Binding Assay, Bioprocessing, Recombinant

    Ability of anti-PAD2 antibodies to inhibit PAD2-mediated citrullination of N α -benzoyl-L-arginine ethyl ester (BAEE). S4, S10, S24, S108, S170, and S309 antibodies were incubated with recombinant human PAD2 (rhPAD2) and buffer (20 mM Tris-HCl (pH 7.6), 150 mM NaCl, and 1 mM dithiothreitol) in a total volume of 44 μ L for 30 min at 25°C. BAEE and CaCl 2 (volume: 6 μ L) were added simultaneously and the mixture was stirred well. The mixture comprised 5 nM rhPAD2, 10 mM BAEE, 1 mM CaCl 2 , and various concentrations of antibodies (0.1–600 nM). The mixture was incubated at 37°C for 4 h, and 12.5 μ L of 5 M perchloric acid was added to stop the reaction. The level of citrullinated BAEE was determined using a colorimetric assay system.

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Ability of anti-PAD2 antibodies to inhibit PAD2-mediated citrullination of N α -benzoyl-L-arginine ethyl ester (BAEE). S4, S10, S24, S108, S170, and S309 antibodies were incubated with recombinant human PAD2 (rhPAD2) and buffer (20 mM Tris-HCl (pH 7.6), 150 mM NaCl, and 1 mM dithiothreitol) in a total volume of 44 μ L for 30 min at 25°C. BAEE and CaCl 2 (volume: 6 μ L) were added simultaneously and the mixture was stirred well. The mixture comprised 5 nM rhPAD2, 10 mM BAEE, 1 mM CaCl 2 , and various concentrations of antibodies (0.1–600 nM). The mixture was incubated at 37°C for 4 h, and 12.5 μ L of 5 M perchloric acid was added to stop the reaction. The level of citrullinated BAEE was determined using a colorimetric assay system.

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Incubation, Recombinant, Colorimetric Assay

    Ability of anti-PAD2 antibodies to inhibit recombinant human PAD2- (rhPAD2-) mediated histone citrullination. The anti-PAD2 antibodies were mixed with rhPAD2 and buffer as described in the legend of at 25°C for 30 min. Histone (1.8 mg/mL) and CaCl 2 (1 mM) were added simultaneously and the mixture was incubated for 4 h at 37°C. Citrullinated histone was determined using a colorimetric assay.

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Ability of anti-PAD2 antibodies to inhibit recombinant human PAD2- (rhPAD2-) mediated histone citrullination. The anti-PAD2 antibodies were mixed with rhPAD2 and buffer as described in the legend of at 25°C for 30 min. Histone (1.8 mg/mL) and CaCl 2 (1 mM) were added simultaneously and the mixture was incubated for 4 h at 37°C. Citrullinated histone was determined using a colorimetric assay.

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Recombinant, Incubation, Colorimetric Assay

    Alanine scanning of epitopes along with their half-maximal effective concentration. EC 50 (ng/mL) values determined using enzyme-linked immunosorbent assay ∗ .

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Alanine scanning of epitopes along with their half-maximal effective concentration. EC 50 (ng/mL) values determined using enzyme-linked immunosorbent assay ∗ .

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Residue